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| Title | Soil Bioassay Pilot Study: Evaluation of the Screening Level Bioassays for Use in Soil Toxicity assessments at Hazardous Waste Sites Under the Model Toxics Control Act | |
| Month-Year Published | August 1993 | |
| Online Availability |
3005 kilobytes, requires version 4.0 or later of Adobe Acrobat Reader Software get Acrobat Reader
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| Short Description |
The Washington State Department of Ecology is developing guidelines for addressing environmental protection at hazardous waste sites under the Model Toxics Control Act (MTCA). Five bioassay protocols being evaluated for use in conducting soil toxicity screening under MTCA were tested under a range of environmental conditions and contaminant concentrations. (Also see abstract below) | |
| Publication Number | 93-e45 | |
| Author(s) | Norton, D. and M. Stinson | |
| Print Availability |
Not maintained in stock. Copy must be made from archive version.
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| Number of pages | 112 pp. | |
| Keywords | bioassay, chemical, contaminant, evaluation, guidelines, hazardous waste, metals, Model Toxics Control Act, pesticides, petroleum, protocol, recommendations, response, responses, site investigation, soil, study, toxicity, toxics | |
| Abstract | Long Description |
The Washington State Department of Ecology is developing guidelines for addressing environmental protection at hazardous waste sites under the Model Toxics Control Act (MTCA). Five bioassay protocols being evaluated for use in conducting soil toxicity screening under MTCA were tested under a range of environmental conditions and contaminant concentrations. The five bioassays evaluated were 1) Daphnia magna- static acute 48-hour, 2) Plant Vigor (Lactuca sativa)- growth, 14-day, 3) Earthworm (Eisenia foetida)- survival 14-day, 4) Fathead Minnow (Pimephales promelas)- static acute 48-hour, and 5) Frog Embryo Teratogenesis Assay Xenopus (FETAX), (Xenopus laevis)- whole embryo static renewal, 96-hour. Soil contaminants included: heavy metals, petroleum products (gasoline and diesel), creosote, and pesticides. Significant toxic responses were obtained with all five of the screening level bioassays. Overall the greatest number of toxic responses to the bioassay suite (percentage of samples tested that exhibited a toxic response for all bioassays) was measured at the creosote contaminated site (42%). Responses to the bioassay suite at the other sites were petroleum products (32%), metals (26%), and pesticides (13%). FETAX was the most sensitive bioassay tested (55% of the samples tested exhibited a toxic response). Comparing the bioassay responses obtained to MTCA residential soil cleanup levels suggests that the established human health standards for the chemicals tested might not provide adequate protection for environmental concerns in several instances. Recommendations for modifying the bioassay protocols were also provided. |
This page last updated August 17, 2011
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