Publication Summary

Title

Earthworm Bioassay Protocol for Soil Toxicity Screening

Month-Year PublishedJune 1996
Online Availability
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Short Description

Earthworm bioassays are a widely recognized tool for evaluating the toxicity of contaminated soils. The earthworm bioassay described in this document is intended to be used in screening level assessments of soil toxicity at hazardous waste sites being investigated under the Washington Model Toxics Control Act Cleanup Regulation.

(Also see abstract below)
Publication Number96-327
Author(s)Norton, D.
Print Availability
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Number of pages 22 pp. + app (35 total)
Keywords assessment, bioassay, Chemistry, cleanup, environmental, Environmental Protection Agency, laboratory, meeting, methods, model, Model Toxics Control Act, protocol, regulation, soil, toxic, toxicity, toxics, waste
Abstract Long Description

Earthworm bioassays are a widely recognized tool for evaluating the toxicity of contaminated soils. The earthworm bioassay described in this document is intended to be used in screening level assessments of soil toxicity at hazardous waste sites being investigated under the Washington Model Toxics Control Act Cleanup Regulation.

In this protocol, earthworms (Eisenia foetida) are added to field collected site soils. The primary test endpoint is earthworm mortality, recorded on day 14. It is not necessary to conduct a dilution series.

The testing procedures described in this document have been adapted from protocols developed by the following groups: United States Environmental Protection Agency Research Laboratory, Corvallis, Oregon; the Commission of the European Communities of the Organization for Economic Cooperation and Development; and documents prepared for the Soil and Plant Toxicity Assessment Short Course at the 12th Annual Meeting of the Society of Environmental Toxicology and Chemistry in November 1991.

This page last updated August 17, 2011