A Citizen's Guide to Understanding and Monitoring Lakes and Streams
Chapter 4 - From the Field to the Lab
Ground Rules
The following ground rules refer to general sampling guidelines that are applicable to
all water quality samples collected. (For each of the parameters described, special
precautions may be needed to ensure collection of a good quality sample. These are
described later for each parameter.
- The single most important ground rule for the monitoring program is this: Document
everything you do. A permanent record lets others who may want to use your results know
how the data were obtained so they can use the same procedures or at least know how to
compare them. Documentation becomes even more important in a volunteer monitoring program
since it provides a ready-made training aid for new volunteers.

- Safety first! Never enter a stream or lake if the current is too strong or conditions
too rough use a safety line in swift waters. Always have a buddy along who can help
if you get in trouble. Wear a life vest whenever you are in a boat.
- All sample containers need to be washed with a dilute acid (sulfuric or hydrochloric)
and thoroughly rinsed with deionized-distilled water. It is very important to keep these
bottles clean between collecting the samples. Keep their caps on tight, and dont
remove them until the sample water is collected. The Puget Sound Estuary Program protocols
and standard methods referenced at the back of this guide give detailed washing
guidelines.
- Rinse each container with sample water three times before placing the sample in the
container permanently. (Some samples require addition of a preservative that may be added
ahead of time. If this is the case, then the bottles should not be rinsed first.)
- When sampling in a stream, extra precautions are needed to ensure a sample is not
collected from a disturbed area. Always begin sampling at the station nearest the mouth of
the stream and work your way upstream.
- When it is necessary to enter a stream to collect a sample, always take a few steps
upstream from where you entered the water, face into the current, lean forward, and reach
upstream to collect the sample.
- Always collect samples from a few inches below the surface of a stream, or from the
depth of about an arms length in a lake.
- When sampling in a lake or over a bridge where equipment can be sunk and lost, tie off
the equipment to a solid, stable object first.
- Label all sample bottles with station name, date, time, and parameters to be analyzed.
Use indelible ink!
- Take lots of field notes. Include weather observations, visual appearance of the
sampling station or water quality, equipment problems, and description of techniques used.
- Be consistent. Dont change sampling method, equipment use, or station locations
between sampling stations or sampling days without noting why the change was made and
permanently identifying which data belongs to which set of sampling techniques or
equipment used.
There are quite a number of techniques for getting water from the lake or stream into a
sample bottle. The one selected depends upon the sampling site.
The most common method is to hand dip the sample. It also is the easiest. Hand dipping
is ideal if youre sampling from a boat and only need a surface sample, or if you can
wade far enough from shore to collect your samples. To avoid capturing the tiny organisms
and debris that float on the surface film of the water, the sample should be collected
from below the water surface. Hand dipping lets you collect water from the right location
and depth.
To collect samples as far from the shoreline as possible (lakes) or well out into the
main moving channel (stream), use a long or extendable pole. Rig the pole so that it can
hold a sample bottle, either temporarily or permanently. Such a device is easy to make and
works well. It also allows you to control the depth at which the sample is collected.
Samples also have been collected by using a weighted bucket attached to a rope. This
method is handy when sampling from a tall bridge where a long pole wont work. If the
water is shallow, make sure the bucket doesnt disturb the stream bottom.
More sophisticated equipment is usually needed to collect samples from more than one
depth in a lake. Several samplers have been designed for this purpose. The most common are
tube shaped samplers with openings on both ends. The ends are propped open like a
mouse trap while the sampler is lowered to the desired depth. The line used to
lower the sampler is marked in 1-foot or 1-meter sections and is equipped with a weight,
called a messenger. When the sampler reaches the desired depth, the messenger is released.
When the messenger hits the sampler, the trap is sprung, the ends close, and the sample
then can be lifted to the surface.
Next Section - Sampling and Measuring
Methods
Return to Table of
Contents | Lakes | Streams | From the Field to the Lab | Hydrology
Last updated on
April 01, 2008
|